Biochem/physiol Actions

Primary TargetFluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

General description

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Manufactured by Daiichi Pure Chemicals Co., Ltd

Other Notes

Berkels, R., et al. 2000. Cell Calcium27, 281.Kojima, H., et al. 1998. Anal. Chem. 70, 2446.Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Packaging

1 mg in Plastic ampoule

Physical form

A 5 mM (1 mg in 550 µl) solution in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Irritant (B)